Urokinase-type plasminogen activator: proenzyme, receptor, and inhibitors

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Urokinase-type plasminogen activator: proenzyme, receptor, and inhibitors

N 'Ew information has recently become available that we believe may be of value for a more precise focusing of research on the role of plasminogen activators (PAs) in cellular physiology and pathology. PAs convert the abundant extracellular zymogen plasminogen into plasmin, an active protease that, directly or indirectly, can promote degradation of all components of the extracellular matrix. Th...

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Plasminogen activator inhibitor from human fibrosarcoma cells binds urokinase-type plasminogen activator, but not its proenzyme.

An approximately 75% pure form of a human Mr approximately 54,000 plasminogen activator inhibitor from conditioned culture fluid of the fibrosarcoma cell line HT-1080 was obtained by a single step of chromatography on concanavalin A-Sepharose. The inhibitor inhibited human urokinase-type plasminogen activator (u-PA) and tissue-type plasminogen activator, but not plasmin. Rabbit antibodies again...

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Uncharged isocoumarin-based inhibitors of urokinase-type plasminogen activator

BACKGROUND Urokinase-type plasminogen activator (uPA) plays a major role in extracellular proteolytic events associated with tumor cell growth, migration and angiogenesis. Consequently, uPA is an attractive target for the development of small molecule active site inhibitors. Most of the recent drug development programs aimed at nonpeptidic inhibitors targeted at uPA have focused on arginino mim...

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Urokinase-type plasminogen activator promotes paracellular transmigration of neutrophils via Mac-1, but independently of urokinase-type plasminogen activator receptor.

BACKGROUND Urokinase-type plasminogen activator (uPA) has recently been implicated in the pathogenesis of ischemia-reperfusion (I/R) injury. The underlying mechanisms remain largely unclear. METHODS AND RESULTS Using in vivo microscopy on the mouse cremaster muscle, I/R-elicited firm adherence and transmigration of neutrophils were found to be significantly diminished in uPA-deficient mice an...

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Dynamic assembly of the urokinase-type plasminogen activator signaling receptor complex determines the mitogenic activity of urokinase-type plasminogen activator.

The urokinase-type plasminogen activator (uPA) receptor (uPAR) functions in concert with co-receptors, including integrins, FPR-like receptor-1/lipoxin A4 receptor, and the epidermal growth factor receptor (EGFR), to initiate cell signaling. uPAR co-receptors may be dynamically organized into a multiprotein signaling receptor complex. In Chinese hamster ovary-K1 (CHO-K1) cells, uPA-binding to u...

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ژورنال

عنوان ژورنال: The Journal of Cell Biology

سال: 1987

ISSN: 0021-9525,1540-8140

DOI: 10.1083/jcb.104.4.801